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              Research
              
              
                            
                            2.  
                            
                            Detection of Mycobacterium avium subsp. 
                            paratuberculosis in asymptomatic bovines by 
                            IS900 polymerase chain reaction - 
                            Remya Raveendran, P. 
                            M. Priya, Koshy John, G. KrishnanNair, K. 
                            Vijayakumar
                            Vet World. 2011; 4(6): 248-249
               
              
              
              doi: 
              10.5455/vetworld.4.248
              
              
               
              
              
              
   
 
              
               
               
               
              
              
              Abstract
              
                              
                            
                            Faecal samples were collected from 58 asymptomatic 
                            bovines and after DNA extraction IS900 Polymerase 
                            Chain Reaction (IS900 PCR) was performed to detect 
                            Mycobacterium avium subsp. paratuberculosis (MAP). 
                            Ten samples (17.24 %) were positive for MAP. The 
                            results indicated that the IS900 PCR assay can be 
                            used for the early diagnosis of bovine 
                            paratuberculosis. 
              
                            
              Keywords: Paratuberculosis, 
                            MAP, IS900 PCR